Q 11 :    

In sea urchin DNA, which is double stranded, 17% of the bases were shown to be cytosine. The percentages of the other three bases expected to be present in this DNA are:   [2015]

  • G 17%, A 33%, T 33%

     

  • G 8.5%, A 50%, T 24.5%

     

  • G 34%, A 24.5%, T 24.5%

     

  • G 17%, A 16.5%, T 32.5%

     

(1)

According to Chargaff’s rule, the amount of adenine is always equal to that of thymine and the amount of guanine is always equal to that of cytosine, i.e., A = T and G = C. Also, the purines and pyrimidines are always in equal amounts, i.e., A + G = T + C. Now, given dsDNA has 17% cytosine and hence guanine will be also 17%. So, A + T must be 66%, therefore, percentage of adenine or thymine would be 66 / 2 = 33%.

 



Q 12 :    

Histones are enriched with -                     [2025]

  • Lysine & Arginine

     

  • Leucine & Lysine

     

  • Phenylalanine & Leucine

     

  • Phenylalanine & Arginine

     

(1)

In eukaryotes, packaging of DNA is much more complex. There is a set of positively charged, basic proteins called histones.

Histones are organised to form a unit of light molecules called histone octamer.

They are rich in the basic amino acid residues lysine and arginine.