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Solution


Q.

Which one of the following statements is not true regarding gel electrophoresis technique                 [2022]
1 The process of extraction of separated DNA strands from gel is called elution.  
2 The separated DNA fragments are stained by using ethidium bromide.  
3 The presence of chromogenic substrate gives blue coloured DNA bands on the gel.  
4 Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.  

Ans.

(3)

In gel electrophoresis, the DNA fragments separate according to their size through the agarose gel, with smaller fragments moving farther away as compared to larger ones. The orange coloured bands of DNA can be visualised by staining them with ethidium bromide followed by exposure to UV radiations. The separated bands of DNA are cut out from the agarose gel is called elution.