Q.

Which of the following restriction enzymes produces blunt ends             [2016]

1 SalI  
2 EcoRV  
3 XhoI  
4 Hind III  

Ans.

(2)

EcoRV is a type II restriction endonuclease isolated from certain strains of E. coli. It creates blunt ends. It recognises the palindromic sequence of 6 bases. SalI, XhoI and Hind III restriction enzymes produce sticky ends.