Which of the following is not a desirable feature of a cloning vector?

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Q.
Which of the following is not a desirable feature of a cloning vector [2022]

1 Presence of origin of replication

2 Presence of a marker gene

3 Presence of single restriction enzyme site

4 Presence of two or more recognition sites

Ans.
(4)

In order to link alien DNA, the vector needs to have preferable single recognition site for commonly used restriction enzyme. Presence of more than one recognition sites within vector will generate several fragments and will complicate the gene cloning.

Solution

Q.
Which of the following is not a desirable feature of a cloning vector [2022]

1 Presence of origin of replication

2 Presence of a marker gene

3 Presence of single restriction enzyme site

4 Presence of two or more recognition sites

Ans.
(4)

In order to link alien DNA, the vector needs to have preferable single recognition site for commonly used restriction enzyme. Presence of more than one recognition sites within vector will generate several fragments and will complicate the gene cloning.

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Solution

Q. Which of the following is not a desirable feature of a cloning vector [2022]

1 Presence of origin of replication

2 Presence of a marker gene

3 Presence of single restriction enzyme site

4 Presence of two or more recognition sites

Ans. (4) In order to link alien DNA, the vector needs to have preferable single recognition site for commonly used restriction enzyme. Presence of more than one recognition sites within vector will generate several fragments and will complicate the gene cloning.

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Q.
Which of the following is not a desirable feature of a cloning vector [2022]

Ans.
(4)

In order to link alien DNA, the vector needs to have preferable single recognition site for commonly used restriction enzyme. Presence of more than one recognition sites within vector will generate several fragments and will complicate the gene cloning.