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Solution


Q.

Which of the following is a restriction endonuclease                 [2016]
1 DNase I  
2 RNase  
3 Hind II  
4 Protease  

Ans.

(3)

Hind II is the first restriction endonuclease. It was isolated from Haemophilus influenzae Rd. It always cuts DNA at specific position producing blunt ends. DNase I is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide. RNase is a type of nuclease that catalyses the degradation of RNA into smaller components. It can be endoribonuclease or exoribonuclease. A protease is an enzyme that performs proteolysis, i.e., protein catabolism by hydrolysis of the peptide bonds.