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Solution


Q.

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)               [2021]
1 Annealing, Denaturation, Extension  
2 Denaturation, Annealing, Extension  
3 Denaturation, Extension, Annealing  
4 Extension, Denaturation, Annealing  

Ans.

(2)

A single PCR cycle involves three basic steps.
(i) Denaturation (DNA is heated to high temperature, usually 94°–96°C)
(ii) Primer annealing (two oligonucleotide primers anneal to each of the single stranded template DNA, temperature usually 40°–60°C) and
(iii) Extension (Taq DNA polymerase synthesises the DNA region between primers, optimum temperature 72°C).