Q.

Which of the following enzyme(s) are NOT essential for gene cloning                            [2025]

A.   Restriction enzymes

B.   DNA ligase

C.   DNA mutase

D.   DNA recombinase

E.   DNA polymerase

Choose the correct answer from the options given below:

 

1 C and D only  
2 A and B only  
3 D and E only  
4 B and C only  

Ans.

(1)

Gene cloning is a process where a specific gene or DNA sequence is isolated and replicated, creating multiple identical copies.

In gene cloning, restriction enzymes, DNA ligase and DNA polymerase are primarily used.