Q.

Thermostable DNA polymerase used in PCR was isolated from                      [2023]

1 Thermus aquaticus  
2 Escherichia coli  
3 Agrobacterium tumefaciens  
4 Bacillus thuringiensis  

Ans.

(1)

In PCR, the repeated amplification is achieved by the use of a thermostable DNA polymerase enzyme Taq polymerase which is obtained from bacterium Thermus aquaticus. This enzyme remains active during the high temperature induced for denaturation of double stranded DNA.