Q.

The following diagram showing restriction sites in E. coli cloning vector or pBR322. Find the role of 'X' and 'Y' genes.       [2024]

1 The gene ‘X’ is responsible for resistance to antibiotics and ‘Y’ for protein involved in the replication of Plasmid.  
2 The gene ‘Y’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of Plasmid.  
3 The gene ‘X’ is for protein involved in replication of Plasmid and ‘Y’ for resistance to antibiotics.  
4 Gene ‘X’ is responsible for recognition sites and ‘Y’ is responsible for antibiotic resistance.  

Ans.

(2)

In the given diagram showing restriction sites in E.coli cloning vector pBR322, X and Y represent ori and rop, respectively. The gene ‘X’ is responsible for controlling the copy number of the linked DNA and ‘Y’ for protein involved in the replication of plasmid.