The correct order of steps in Polymerase Chain Reaction (PCR) is:

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Q.
The correct order of steps in Polymerase Chain Reaction (PCR) is: [2018]

1 extension, denaturation, annealing

2 annealing, extension, denaturation

3 denaturation, extension, annealing

4 denaturation, annealing, extension

Ans.
(4)

A single PCR amplification cycle involves three basic steps, denaturation heating of target DNA to high temperature resulting in separation of two strands, annealing two oligonucleotide primers anneal or hybridise to each single template DNA and extension. Taq DNA polymerase synthesises DNA between primers and primers extend towards each other such that DNA stranded segment lying between the two is copied.

Solution

Q.
The correct order of steps in Polymerase Chain Reaction (PCR) is: [2018]

1 extension, denaturation, annealing

2 annealing, extension, denaturation

3 denaturation, extension, annealing

4 denaturation, annealing, extension

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Solution

Q. The correct order of steps in Polymerase Chain Reaction (PCR) is: [2018]

1 extension, denaturation, annealing

2 annealing, extension, denaturation

3 denaturation, extension, annealing

4 denaturation, annealing, extension

Want Us to Email you About Special Offers & Updates?

Q.
The correct order of steps in Polymerase Chain Reaction (PCR) is: [2018]