Q.

Ten E. coli cells with N15-dsDNA are incubated in medium containing N14 nucleotide. After 60 minutes, how many E. coli cells will have DNA totally free from N15      [2022]

1 20 cells  
2 40 cells  
3 60 cells  
4 80 cells  

Ans.

(3)

E. coli divides in 20 minutes. After 60 minutes, generation III would have 75% DNA completely free from N15 and 25% of DNA would be hybrid N14/N15. So, out of 80 cells formed, 60 cells will be free from N15 DNA.