Q.

The shape of guard cells changes due to change in the:

1 protein composition of cells.  
2 temperature of cells.  
3 amount of water in cells.  
4 position of nucleus in cells.  

Ans.

(3)

The amount of water in guard cells causes change in their shape. When water enters into the guard cell, it causes the stomatal pore to open. Similarly, if the guard cells shrink as a result of water loss, the pore closes.