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Solution


Q.

Regarding catalytic cycle of an enzyme action, select the correct sequential steps.

A. Substrate enzyme complex formation.
B. Free enzyme ready to bind with another substrate.
C. Release of products.
D. Chemical bonds of the substrate broken.
E. Substrate binding to active site.

Choose the correct answer from the options given below:                                [2024]
1 E, A, D, C, B  
2 A, E, B, D, C  
3 B, A, C, D, E  
4 E, D, C, B, A  

Ans.

(1)

The catalytic cycle of an enzyme action can be described as follows:

(i) First, the substrate binds to the active site of the enzyme, fitting into the active site. This leads to the formation of substrate enzyme complex. The binding of the substrate induces the enzyme to alter its shape, fitting more tightly around the substrate.

(ii) The active site of the enzyme, now in close proximity of the substrate breaks the chemical bonds of the substrate and the new enzyme-product complex is formed.

(iii) The enzyme releases the products of the reaction and the free enzyme is ready to bind to another molecule of the substrate and run through the catalytic cycle once again.