Plasmid pBR322 has PstI restriction enzyme site within gene that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E. coli strain.

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Q.
Plasmid pBR322 has PstI restriction enzyme site within gene ${amp}^{R}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $β$ -galactoside production and the recombinant plasmid is inserted in an E. coli strain. [2021]

Solution

Q.
Plasmid pBR322 has PstI restriction enzyme site within gene ${amp}^{R}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $β$ -galactoside production and the recombinant plasmid is inserted in an E. coli strain. [2021]

1 It will be able to produce a novel protein with dual ability.

2 It will not be able to confer ampicillin resistance to the host cell.

3 The transformed cells will have the ability to resist ampicillin as well as produce $β$ -galactoside.

4 It will lead to lysis of host cell.

Ans.
(2)

(2)

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Solution

Q. Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E. coli strain. [2021]

1 It will be able to produce a novel protein with dual ability.

2 It will not be able to confer ampicillin resistance to the host cell.

3 The transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.

4 It will lead to lysis of host cell.

Ans. (2)

(2)

Want Us to Email you About Special Offers & Updates?

Q.
Plasmid pBR322 has PstI restriction enzyme site within gene ${amp}^{R}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $β$ -galactoside production and the recombinant plasmid is inserted in an E. coli strain. [2021]

3 The transformed cells will have the ability to resist ampicillin as well as produce $β$ -galactoside.

Ans.
(2)