Q.

Oxidative phosphorylation is                  [2016]

1 formation of ATP by transfer of phosphate group from a substrate to ADP  
2 oxidation of phosphate group in ATP  
3 addition of phosphate group to ATP  
4 formation of ATP by energy released from electrons removed during substrate oxidation.  

Ans.

(4)

Oxidative phosphorylation is the synthesis of energy-rich ATP molecules with the help of energy liberated during oxidation of reduced co-enzymes (NADH, FADH2) produced in respiration.

The enzyme required for this synthesis is called ATP synthase. It is located in F1 or head piece of F0F1 or elementary particles present in the inner mitochondrial membrane. F1 particle is capable of ATP synthesis.

ATP synthase becomes active in ATP formation only when there is a proton gradient having higher concentration of H+ or protons on the F0 side as compared to F1 side. This higher concentration creates an electric potential across the mitochondrial membrane. The proton gradient and membrane electric potential together form proton motive force (PMF). The flow of protons through the F0 channel induces F1 particle to function as ATP synthase. The energy of the proton gradient is used in attaching a phosphate radical to ADP by high-energy bond. This produces ATP.