Q.

Which of the following limits the number of trophic levels in a food chain

1 Decrease in energy at higher trophic levels  
2 Deficient food supply  
3 Polluted air  
4 Water  

Ans.

(1)

A considerable amount of energy is used to keep organisms alive at each trophic level. As an organism progresses through the trophic levels, it receives less and less energy. The number of trophic levels is restricted to 3–4 since the energy available for the next level is insufficient to keep the organisms alive after that.