Q.

Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322               [2023]

1 Pst I  
2 Pvu I  
3 EcoR I  
4 BamH I  

Ans.

(4)

In the plasmid vectors, pBR322, tetracycline resistance gene contains recognition sites for two restriction enzymes BamH I and Sal I. The recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA at one of these sites.