Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322 [2023]
(4)
In the plasmid vectors, pBR322, tetracycline resistance gene contains recognition sites for two restriction enzymes BamH I and Sal I. The recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA at one of these sites.