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Solution


Q.

In vitro clonal propagation in plants is characterized by:                   [2014]
1 PCR and RAPD  
2 Northern blotting  
3 electrophoresis and HPLC  
4 microscopy  

Ans.

(1)

Clonal propagation can be characterized by PCR and RAPD. The polymerase chain reaction (PCR) technique, generates microgram (µg) quantities of DNA copies (upto billion copies) of the desired DNA (or RNA) segment, present even as a single copy in the initial preparation, in a matter of few hours. RAPD stands for Random Amplification of Polymorphic DNA. It is a type of PCR, but the segments of DNA that are amplified are random. No knowledge of the DNA sequence for the targeted gene is required, as the primers will bind somewhere in the sequence, but it is not certain exactly where. Its resolving power is much lower than targeted, species specific DNA comparison methods, such as short tandem repeats.