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Solution


Q.

Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.              [2019]
1 The enzyme recognises a specific palindromic nucleotide sequence in the DNA.  
2 The enzyme cuts DNA molecule at identified position within the DNA.  
3 The enzyme binds DNA at specific sites and cuts only one of the two strands.  
4 The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.  

Ans.

(3)

The restriction endonuclease enzyme inspects the length of a DNA sequence. Once it recognises specific sequence, it binds to the DNA and cuts each of the two strands of the double helix at specific points in their sugar phosphate backbone. Special sequence in the DNA recognised by restriction endonuclease is called palindromic nucleotide sequence.