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Assisted reproductive technologies (ART) include a number of special techniques which assist infertile couples to have children. An important technique of ART is test tube baby programme. The baby produced by conceiving in a culture dish and nursing in the uterus is called a test tube baby. This method involves in vitro fertilisation (IVF), i.e., fertilisation of male and female gamete outside the body in almost similar conditions as that in the body followed by embryo transfer (ET). Zygote or embryo upto 8 blastomeres is transferred into the fallopian tube (ZIFT – Zygote Intra Fallopian Transfer) and mature embryo with more than 8 blastomeres is transferred to uterus to complete its further development.