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Solution


Q.

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using          [2016]
1 EcoRI  
2 Taq polymerase  
3 polymerase III  
4 ligase  

Ans.

(4)

Ligase is a class of enzymes that catalyse the formation of covalent bonds using the energy released by the cleavage of ATP. Ligases are important in the synthesis and repair of many biological molecules, including DNA ligase and used in genetic engineering to insert foreign DNA into cloning vectors.