Which one is the correct product of DNA dependent RNA polymerase to the given template? [2024]
3′ TACATGGCAAATATCCATTCA 5′
5′ AUGUACCGUUUAUAGGUAAGU 3′
5′ AUGUAAAGUUUAUAGGUAAGU 3′
5′ AUGUACCGUUUAUAGGGAAGU 3′
5′ ATGTACCGTTTATAGGTAAGT 3′
Match List I with List II. [2024]
| List I | List II | ||
| A. | RNA polymerase III | (I) | snRNPs |
| B. | Termination of transcription | (II) | Promotor |
| C. | Splicing of exons | (III) | Rho factor |
| D. | TATA box | (IV) | SnRNAs, tRNA |
Choose the correct answer from the options given below:
A-II, B-IV, C-I, D-III
A-III, B-II, C-IV, D-I
A-III, B-IV, C-I, D-II
A-IV, B-III, C-I, D-II
A transcription unit in DNA is defined primarily by the three regions in DNA and these are with respect to upstream and downstream end. [2024]
repressor, operator gene, structural gene
structural gene, transposons, operator gene
inducer, repressor, structural gene
promotor, structural gene, terminator
(4)
In transcription unit, the promoter and terminator flank the structural gene. The promoter is said to be located towards 5′-end (upstream) of the structural gene and the terminator towards the 3′-end (downstream) of the coding strand.
Given below are two statements: [2023]
Statement I: The process of copying genetic information from one strand of the DNA into RNA is termed as transcription.
Statement II: A transcription unit in DNA is defined primarily by the three regions in the DNA i.e., a promotor, the structural gene and a terminator.
In the light of the above statements, choose the correct answer from the options given below:
Statement I is true but statement II is false.
Statement I is false but statement II is true.
Both statement I and statement II are true.
Both statement I and statement II are false.
What is the role of RNA polymerase III in the process of transcription in Eukaryotes? [2023]
Transcription of precursor of mRNA
Transcription of only snRNAs
Transcription of rRNAs (28S, 18S and 5.8S)
Transcription of tRNA, 5 SrRNA and snRNA
(4)
RNA polymerase III is responsible for transcription of tRNA, 5SrRNA and snRNAs (small nuclear RNAs). RNA polymerase I transcribes rRNA (28S, 18S and 5.8S) whereas RNA polymerase II transcribes precursor of mRNA, the heterogeneous nuclear RNA (hnRNA).
Which one of the following is the sequence on corresponding coding strand, if the sequence on mRNA formed is as follows: [2023]
5′ AUCGAUCGAUCGAUCGAUCGAUCGAUCG 3′
5′ ATCGATCGATCGATCGATCGATCGATCG 3′
3′ ATCGATCGATCGATCGATCGATCGATCG 5′
5′ UAGCUAGCUAGCUAGCUAGCUAGCUAGC 3′
3′ UAGCUAGCUAGCUAGCUAGCUAGCUAGC 5′
What is the role of RNA polymerase III in the process of transcription in eukaryotes? [2021]
Transcribes only snRNAs
Transcribes rRNAs (28S, 18S and 5.8S)
Transcribes tRNA, 5S rRNA and snRNA
Transcribes precursor of mRNA
Identify the correct statement. [2021]
Split gene arrangement is characteristic of prokaryotes.
In capping, methyl guanosine triphosphate is added to the 3′ end of hnRNA.
RNA polymerase binds with Rho factor to terminate the process of transcription in bacteria.
The coding strand in a transcription unit is copied to an mRNA.
(3)
Split gene arrangement is a characteristic of eukaryotes. In capping, an unusual nucleotide (methyl guanosine triphosphate) is added to the 5′-end of hnRNA. RNA polymerase binds with termination factor Rho () to terminate the process of transcription in bacteria. The coding strand does not code for anything.
Which is the “only enzyme” that has “capability” to catalyse initiation, elongation and termination in the process of transcription in prokaryotes? [2021]
DNase
DNA dependent DNA polymerase
DNA dependent RNA polymerase
DNA Ligase
(3)
In bacteria, there is a single DNA dependent RNA polymerase that catalyses transcription of all types of RNA. It associates transiently with initiation factor () and termination factor () to carry out initiation and termination of transcription respectively. It catalyses the elongation as its uses nucleoside triphosphates as substrates and polymerises in a template dependent fashion as rule of complementarity.
Name the enzyme that facilitates opening of DNA helix during transcription. [2020]
DNA ligase
DNA helicase
DNA polymerase
RNA polymerase
